89d3865b483ca694fbb6efece0f3c6f3

2024-02-01

12.2 MB

RAR Archive

Functional genomic annotation was performed using three tools, for increased robustness, as being the three most used tools available for functional annotation: i) eggNOG-mapper v.5.0 (Jensen et al. 2008); ii) kofamKOALA v. 2022-04-03 (Aramaki et al. 2020); iii) KAAS-KEEG Automatic Annotation Server (Moriya et al. 2007). From each software, a list of annotated KO´s (KEEG Orthology), as representing the functional orthologs, was obtained, and an in-house script was built in Python to obtain the list of Consensus KO´s for each genome. The script would search for a consensus annotation in a way that: (i) if only a platform would provide a KO, that one would be considered; (ii) if two platforms would provide a KO and the third a different one, the KO that appeared in the two matching results was selected as consensus; (iii) if three different KO´s were obtained (or two different KO´s and a third with no result), it would be considered undefined.

2024-01-30